Download this application note from Thermo Fisher Scientific to discover an approach using the Neon NxT system that researchers can follow for optimal transfection results ... hypoxic and physioxic ...

1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...

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Furthermore, the transfection procedures used to ... but far greater culture volumes are possible with cells floating free in suspension. To address this, many teams have developed various ...

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