Our basic Confocal Laser Scanning System includes an electronics control unit, an expandable dual PMT module, a 16-position pinhole wheel, a two-channel (488 nm & 642 nm) laser source, and the ...

While confocal microscopy uses a pinhole to reject out-of-focus light to generate the optical section, a multi-photon (or 2-photon) microscope uses a pulsed infrared laser to stimulate fluorescence ...

This 3D-printed confocal laser scanning microscope is a ... and a photodiode detector behind a pinhole. The detector feeds a homebrew trans-impedance amplifier that captures data at millions ...

In confocal microscopy, the optical-sectioning ability is created by placement of a field stop, the so-called pinhole, in a conjugate image plane in front of a detector along the fluorescence beam ...

Deconvolution of optimally acquired image stacks enables some improvement in resolution and when combined with closing of the confocal pinhole on CLSM systems or additional optics on a spinning disk ...

Soon, he picked up a side project: developing one of the first confocal fluorescent microscopes. Stelzer and his colleagues designed a device with a pinhole that filtered out-of-focus layers of laser ...

The LSM 9 family with Airyscan 2 from ZEISS provides more options to enable the perfect balance of speed and resolution for today’s confocal-imaging needs. By combining the pinhole-plane imaging ...

A high-performance spinning disk confocal in an enclosed ... There are dual Nipkow disks with 50 or 25-micrometer lens pinhole arrays. The stage is motorized in X, Y, Z and coupled with Olympus’ ...

The Airyscan PrincipleA classic confocal microscope illuminates one spot on the sample to detect the emitted fluorescence signal. Out-of-focus emission light is rejected at a pinhole, the size of ...