Cytosolic delivery of small interfering RNA (siRNA) remains challenging, and a profound understanding of the cellular uptake and intracellular processing of siRNA delivery systems could greatly ...

EV transcytosis is typically an active process, as EVs rarely cross an intact BBB at low temperatures. 61 There are four main mechanisms of transcytosis: clathrin-mediated endocytosis, ...

Results Here, a genome-wide in vivo CRISPR screen identified a novel immune modulator-ADCY7. The transmembrane protein ADCY7 undergoes subcellular translocation via caveolae-mediated endocytosis and ...

To do so, we incubated Cav1KO and Cav1WT MEFs with anti-active beta1-Alexa 488 antibody for 1 hour at 4 o C followed by 3 minutes endocytosis at 37 o C. Immediately after that, we challenged cells for ...

Caveolae-mediated endocytosis and subsequent transcytosis is regulated by both the phosphorylation and interaction of caveolar coat proteins and proteins located at the neck region. PV1, EHD2, and ...

Cavin3 associates with BRCA1 upon disassembly of caveolae in response to mechanical and non-mechanical stressors to influence DNA repair and apoptosis. Skip to Content. eLife home page. ... (2012) ...

More recently, viral entry mediated by non-clathrin non-caveolae endocytosis in lipid rafts has been especially characterized in adeno-associated virus 2 (AAV2) infection ().AAV2 particles enter host ...

These results indicate that the GM3BP-avidin complex bind to GM3 in membrane raft, and are taken into cell through caveolae/raft-mediated endocytosis. The GM3BP-avidin complex was transported into ...

However, upon injection into the blood stream or in a local tissue environment, NP efficacy is determined in part by how they are processed by cells. Most NPs are taken up by cells through one of the ...

To provide direct evidence of caveolae-mediated endocytosis, we generated stable t cells with deficient expression levels of caveolin-1 (denoted “CAV1-DF”) by lentiviral transfection of C166 cells ...

Independently inhibiting clathrin- and caveolae-mediated endocytosis, as well as macropinocytosis significantly decreased intracellular delivery of 4.4- to 500-kDa dextrans. Furthermore, 3D ...